Using the 18-55mm, the Mrs. gets some activity from the auto-focus motor, but no actual results. She is fine with manual focusing and uses the [Live View] most of the time doing her macro work/shots. I tried the 20mm tube with my 70-300mm and nothing .. some activity, but nothing comes in focus at all. I cannot even manually focus on anything.
Michael
The 70-300mm lens is not a fast lens to begin with, and to use an extension tube with it will cut down the amount of light even further. I *think* that may be causing your problem. You mentioned an 85mm macro lens...generally macros are faster...around f/2.8...so when the light is cut down, there is still enough there for the camera to operate its AF.