Check the sharpness of focus
. If you have a scale, then prop it up at an angle of 45 degrees (approx). The top away from you, the bottom towards you,
. Put the camera on the tripod, and level it so that the centre of the lense is around the middle.
. Use the largest aperture, f2.8 in your case.
. Focus the camera on a full graduation, say 6 inches or 15 cm graduation. It is best to adjust the camera so that it is level.
. Now shoot.
You can also verify AF similarly.
. Load the image in computer and examine the sharpness of graduations.
. If the graduation you focused on is the sharpest, then your focus is perfect.
. If the graduation above the mark is sharpest, then you are focusing is at the back.
. If the graduation below the mark is sharpest, then you are focusing is at the front.
For AF adjust the AF fine tune for this lense. The amount can be found on the net.
For MF, adjust the Diopter wheel; which is on the eye piece, and try a few times till you get it right.
NOTE that if your eyes/glasses are outside the range of the diopter adjuster, then you will never get perfect focus in MF. In that case an additional diopter corrected eyepiece is required. some thing like this
DK-20C -2.0 Correction Eyepiece from Nikon
I have the same problem and with my glasses the diopter correction is just out side the range, so where I find the sharpest focus, is way off.