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Message: <blockquote data-quote="Vixen" data-source="post: 383899" data-attributes="member: 21839">Don't forget that with such a small DOF with the macro lens, unless your subject is all on the same plane ie same distance from camera, you will get some areas not in focus. Maybe the flower was not dead square on to the camera? Also...long exposure means ANY air movement may cause some out of focus areas with delicate objects, smoke etc.Also....did you turn IS/VR off?But looking at the pic I'd say not square on to the camera <img src="data:image/gif;base64,R0lGODlhAQABAIAAAAAAAP///yH5BAEAAAAALAAAAAABAAEAAAIBRAA7" class="smilie smilie--sprite smilie--sprite2" alt=";)" title="Wink    ;)" loading="lazy" data-shortname=";)" /></blockquote>