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Message: <blockquote data-quote="WayneF" data-source="post: 304987" data-attributes="member: 12496">It does look like a hair, and it is on the digital sensor, not on the lens.  Your f/stop data (except first value) reinforces that it is on the sensor.  The difference seen is not about depth of field, but instead about that f/25 is a tiny pinpoint  light source, and f/5.6 is a wide light source, which is illuminating the hair on the focal plane.  A wide light makes a wide shadow, which makes it appear more vague. Dust and stuff is best seen at larger f/stop numbers.Use the CLEANING menu in the camera, which will open the shutter and allow you to see the sensor,  D7000 manual, page 284.  Then use like a Rocket blower to blow the hair and dust off.</blockquote>